The Poseidon Select E-chip family of sample supports provides an unparalleled level of flexibility for your liquid in situ experiments. Each Poseidon experiment utilizes a small and large E-chip that form the bottom and top of the closed-cell design, respectively. Each E-chip integrates a durable, 50 nm amorphous silicon nitride window to safely contain liquid with minimum beam scatter. To accommodate the wide variety of samples used in the materials and life sciences markets, the E-chip platform provides the user the ability to specify three key parameters (explained below) to optimize Poseidon for your sample and experiments.
Window Size and Orientation
Poseidon allows you to select from multiple window sizes to provide different viewing areas. The small square E-chip can be positioned such that its window is parallel to or perpendicular to the large E-chip window. Positioning the E-chip windows perpendicular to one another (perpendicular configuration) yields a smaller viewing area but is simpler to align and provides a uniform liquid thickness at each corner of the window.
Spacer Height
Poseidon allows you to control the thickness of the liquid layer by selecting the height of a spacer found on the bottom E-chips. This spacer sets the minimum spacing between the two E-chips, and the spacer height should be chosen so that it’s approximately the same thickness as your samples in order to maximize imaging resolution. For example, scientists studying whole cells may want to choose micron-scale spacers, while those studying nanoparticles may desire a much thinner liquid layers (50nm). Spacers are located on the bottom E-chips only.
Flow or Static Liquid Environment
In addition to specifying the window size and the height of the spacer, you can also specify spacer shape. This enables you to contain your sample in either a closed, static environment or an open, flowing environment. All of the spacer materials and the amorphous silicon nitride windows are robust to most liquids and sample preparation techniques.
A quantity of 1 of any part number indicates 1 box of E-chips. Each box contains 10 E-chips.
**Note: One top and one bottom E-chip are required for every experiment. The spacer that sets the liquid thickness is located on the BOTTOM E-chip. The top E-chip does not contain a spacer for standard liquid experiments.
Key Measurements:
• Length: 2 mm
• Width: 2 mm
• E-chip Thickness: 300 μm
• Silicon Nitride Window Thickness: 50 nm
All feature dimensions listed should be considered nominal. Variations in dimensions can occur.